Magnesium ascorbyl phosphate

Han L, et al. Journal of Hazardous Materials, 2023, 448, 130860.

This study demonstrates the effective use of magnesium ascorbyl phosphate (MAP) in remediating Cr(VI)-contaminated soil through a biochemical process catalyzed by phytase. MAP was applied at 5 wt% of soil, while phytase was added at 1 vol% of soil water. Under an optimal moisture content of 16.8%, MAP was enzymatically hydrolyzed, releasing ascorbic acid (AA) and forming MgHPO₄·3H₂O. AA reduced Cr(VI) to Cr(III), which subsequently precipitated as Cr(OH)₃ and CrPO₄. Over 90% of 500 mg/kg Cr(VI) was reduced. Simultaneously, MgHPO₄·3H₂O filled soil pores, significantly increasing unconfined compressive strength by over twofold. This dual-function strategy offers a novel enzymatically induced phosphate precipitation (EIPP) method for simultaneous Cr(VI) detoxification and soil stabilization using MAP.

Zhang Z, et al. Chemical Engineering Journal, 2023, 472, 145061.

A GelMA-based hydrogel incorporating magnesium ascorbyl phosphate (MAP) was developed to evaluate its multifunctional role in cranial bone defect repair. MAP served as a calcium-free phosphorus source and was uniformly encapsulated into the hydrogel matrix to ensure sustained release. In vitro studies demonstrated MAP enhanced mineralization of BMSCs by promoting Ca²⁺ uptake and reducing oxidative stress through ROS scavenging. Additionally, MAP activated ERK/AKT signaling pathways and promoted angiogenesis, crucial for microenvironmental remodeling. In vivo, a critical-sized cranial defect model in rats revealed that GelMA/MAP hydrogels achieved significantly higher new bone volume (32.74 ± 0.62%) after 8 weeks compared to controls (7.08 ± 0.29%). This study highlights the utility of MAP as a triple-functional agent, offering anti-oxidation, osteoinduction, and pro-angiogenesis in a single injectable platform for bone tissue engineering.

Lakra R, et al. International Journal of Biological Macromolecules, 2021, 166, 333-341.

Magnesium ascorbyl phosphate (MAP) was experimentally evaluated for its ability to stabilize collagen matrices in wound healing. Collagen fibrillation assays demonstrated that MAP accelerated self-assembly kinetics without altering secondary protein structures. Rheological studies indicated a MAP-induced increase in shear viscosity under variable shear rates, suggesting enhanced structural interactions. Differential scanning calorimetry revealed a significant rise in the denaturation temperature of MAP-treated collagen films, while tensile testing confirmed improved Young's modulus, indicating greater mechanical stability. In vivo wound healing was assessed using a rat excisional model, where MAP-stabilized collagen films achieved complete wound closure by day 16. Histological and mechanical analyses of healed skin confirmed full restoration of tensile strength and elasticity, comparable to healthy dermal tissue. These findings highlight MAP's efficacy in enhancing the physicochemical properties of collagen, positioning it as a promising agent for regenerative wound healing applications.

Zhang Z, et al. Biomaterials Advances, 2024, 158, 213779.

A bioactive hybrid hydrogel incorporating magnesium ascorbyl phosphate (MAP) was engineered to improve diabetic wound healing by modulating oxidative stress and enhancing tissue regeneration. The gelatin-based scaffold was fabricated via physical crosslinking and evaluated for its mechanical strength, biocompatibility, and antioxidant activity. In vitro assays confirmed ROS scavenging and promotion of endothelial cell migration. In vivo, full-thickness wounds were induced in diabetic mice and treated with the MAP-loaded hydrogel. Histological analyses revealed accelerated wound closure, enhanced angiogenesis (CD31, VEGF), collagen remodeling (COL-1), and neurogenesis (CGRP). Network pharmacology suggested MAP modulated extracellular matrix remodeling and anti-apoptotic pathways. The scaffold demonstrated significant improvement in tissue repair outcomes, highlighting MAP's therapeutic potential in chronic wound treatment.